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Regarding the samples that scored negative in Kit A while positive or doubtful in Kits B and C, one could also consider that the difference may be due to a non specific inhibitory character in the sera or a possible difference in available epitopes for reaction between the two ELISA methods.
Further information regarding the sample or the experimental conditions can be found in ref. 20.
A note regarding the sample is warranted.
However, for three studies, there were less details regarding the characteristics of the sample that either dropped out or had missing data compared with the final sample population.
PCA is an unsupervised method in the sense that no considerations are made regarding the samples for building the model.
Ethnicity information regarding the samples was unavailable.
For statistics, all the samples that expressed CD44v6 form to were regards as positive.
Heterogeneity and small sample sizes precluded conclusions regarding the interventions that are likely to prevent postoperative delirium.
Sentinel sites notified the CPHRL regarding samples that were collected and ready for pick-up.
Regarding the comment that the consecutive sampling method was already introduced in the 2002 publication [ 23]: We would like to mention, that the original paper described only the principle and justification of the approach.
This pilot study offers guidance regarding the size of tissue sample that is required to account for heterogeneity in the specific biomarkers studied.
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