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The five technical replicates (see Section Transcriptomic RNA seq dataset) are processed independently with regard to each tool and evidence measure.
Results are reported as an average ± SEM (see figure legends for specific details regarding the number of biological replicates, independent experiments and technical replicates).
To address this, we analyzed the technical replicate data regarding what fraction of total targeted nucleotides were subject to stochastic genotypeability and sequencing given comparable, equal input sequence data (Fig. 2a).
from three technical replicates.
All sample are biological replicates rather than technical replicates.
Each biological replicate was read in 2 technical replicates (TR).
from the mean of technical replicates (n=3).
Every qPCR experiment was run in triplicates (n=3 technical replicates).
(a) Unsupervised hierarchical clustering was performed for individual technical replicates.
Data is the mean ± SD of two technical replicates.
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