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A sample was regarded positive for BRSV if the cycle threshold (Ct) was below 45 for both parallels following the manufacturer's recommendation.
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Finally there were questions requesting open-ended comments regarding positive experiences and areas for improvement.
Trace haematuria was regarded as negative and anything above trace was regarded as positive for microhaematuria.
Seven of the cases were clinically regarded as positive for HER2 (2+ or 3+) and three were regarded as negative (0 or 1+).
Only HER2 3+ tumours were regarded as positive for overexpression.
Tumors that were immunochistochemically scored as 3+, or 2+ with FISH-positive, were regarded as positive for HER2 status.
For this reason all samples that showed amplification were regarded as positive for these genes.
A titer ≥20 was regarded as positive for the serotype-specific neutralizing antibodies.
Consequently, 88 children were regarded as positive for celiac disease associated antibodies.
Staining of mitotic figures was ignored and only nuclear staining was regarded as positive for P53.
Cells with blue granules in the nucleus were regarded as positive for TUNEL.
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