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Importantly, when removed from the presence of the anergy-inducing stimulus, anergic cells may regain responsiveness.
To confirm that the observed migratory phenotypes were TβRII dependent, TβRII KO epithelial cells were reconstituted with functional TβRII (RII) [ 37] to regain responsiveness to TGF-β signaling.
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According to Austrian law, informed consent of unresponsive patients was obtained from them after they regained responsiveness.
There was no correlation between the duration of sedation prior to discontinuation and the time to regaining responsiveness.
The written consent of unresponsive patients was obtained either from them after they regained responsiveness or from their next of kin.
There was no correlation between the presence of hepatic or renal dysfunction and the time to regaining responsiveness after discontinuation of sedation.
The definition of chronic ESA hyporesponsiveness was based on the consideration: after how many months of consecutive hyporesponsiveness is it unlikely that patients will subsequently regain ESA responsiveness?
In our study, inhibition of prostaglandin synthesis by application of 28 μM indomethacin, acutely inhibited vasodilation during low oxygen, but after ~2-3 minutesteadyady state) the arterioles had regained their responsiveness, and dilated to low oxygen with a response comparable to the control.
However, after a period of ~2-3 minutesteadyady state) with presence of indomethacin, the vessel regained its responsiveness to low oxygen by dilating again.
In both series of experiments the vessels regained full responsiveness to changes in oxygen tension following wash-out of the drugs.
For unknown reasons, the uterus eventually regains its responsiveness and oxytocin induced prostaglandin production is again possible, finally resulting in luteolysis and oestrus [ 27, 29].
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