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Refrigerated samples were allowed to equilibrate to room temperature and any condensation was allowed to evaporate before scanning.
The molecular composition of raw milk (RM) was assessed for refrigerated samples, which were prepared by diluting 0.01 mL of milk with 0.99 mL of deuterated (99.8%D2O/H2OO, Armar Chemicals) phosphate buffer (90 mM, pH 6.5).
The Pittsburgh business unit then sent the refrigerated samples to the appropriate Nichols site.
Lactate dehydrogenase (LDH) was measured within 24 hr on refrigerated samples with a COBAS MIRA Plus analyzer (Roche Diagnostics, Indianapolis, IN) using kits from Roche.
For the samples stored in DMEM at 37 °C, we observed a significant reduction of RCI compared with the fresh and refrigerated samples (54.4 ± 1.76 %; p ≤ 0.001).
Even though samples were collected as clean as possible, changes in lactate and citrate were identified in the refrigerated samples, which suggest microbial contamination.
Similar(6)
After sample collection, women recorded the collection time on the tube and in their diary, and were asked to refrigerate samples until pickup, after which they were stored at −70°C until shipment to Dresden, Germany on dry ice.
Women were asked to refrigerate samples until pickup on the third day after which they were stored at -70° C until shipment on dry ice to the laboratory of Dr. Clemens Kirschbaum in Dresden, Germany for assay.
Thus, for consistency, we refrigerated all samples at 4 °C until analysis.
In the laboratory, samples were refrigerated until all samples for a participant had been provided.
The milk samples were refrigerated immediately after sampling.
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