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In some of the naturally selected mosquito refractory strains these responses may result in the complete blockage of parasite development.
Thus naturally evolved and genetically selected refractory strains are important for the study of mechanisms that mediate Plasmodium killing [7], [8], [9].
Higher level of NOS during Plasmodium infection, further indicate that refractory strains are under chronic state of hostile cyto-toxic stress because of the production of reactive cytotoxic free radicals viz NO2-, NO3- etc.
New culture protocols now permit the derivation of ESCs from refractory strains (Ying et al, 2008).
Both findings are apparently interrelated because refractory strains are usually the strains with high MLC values.
The destruction of the parasites in Anopheles refractory strains has also been associated with a fitness cost [ 60, 61].
Similar(52)
We hypothesize that upregulation of mosquito innate cytotoxicity due to NOS in refractory strain to Plasmodium vivax infection may contribute to natural refractoriness in An. culicifacies mosquito population.
Dietary feeding of L-NAME along with blood meals significantly inhibited midgut AcNOS activity leading to an increase in oocyst production in An. culicifacies species B. We hypothesize that upregulation of mosquito innate cytotoxicity due to NOS in refractory strain to Plasmodium vivax infection may contribute to natural refractoriness in An. culicifacies mosquito population.
However, we have also observed a significant increase in the live oocyst density in the refractory strain when fed on infected blood meal supplemented with L-NAME.
The iso-female line that was identified as sibling species B and designated as P. vivax refractory strain was established as described by Adak et al [17].
gambiae strains, the L3-5 refractory strain that was selected to melanize Plasmodium, and the An.
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