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It should be noted that the microarray data reflects expression patterns of RPCs in all cell cycle phases, not just S phase, so an exact match of the percentage of RPCs positive for a given gene in the microarray analysis would not necessarily be expected in the [3H]-thymidine/DISH experiments.
This probably reflects expression of Mnx1, which is the next gene past Nom1.
The current sex-biased expression of retrogenes without parental gene does not necessary reflects expression levels when duplication occurred.
Interestingly, gene expression in peripheral unsorted blood accurately reflects expression of the same genes in atherosclerotic arteries [ 12].
The gene expression assay used for progesterone receptor (Mm00435625) did not distinguish between the two isoforms; thus, quantification reflects expression of both PR-A and PR-B.
We therefore infer that the detection of CRP4 transcripts in flowers of non-transgenic plants reflects expression of the endogenous CRP4 gene in synergid cells.
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In this work, the data for the analysis reflect expression values of genes whose dynamics are affected by perturbation experiments.
These qRT-PCR results demonstrated that the microarray analysis accurately reflected expression levels of the miRNAs.
The resulting counts of small RNA sequences detected ideally reflect expression levels of individual microRNAs.
Therefore, antibody-based analysis of V3C18 does not necessarily reflect expression, as detection can be influenced by post-translational processing.
Concomitantly, mCherry expression was slightly reduced in the cells, reflecting expression of one or more of the differentiation specific miRNAs miR-223, miR-155, or miR-142-3p miR-142-3p miR-142-3p
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