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If a detector is used to capture the reflected back light with a reflection setup, a peak resonance wavelength will be shown on the spectrum.
The fiber tip sensor with reflection setup is very easy to use with no alignment needed, and can be used for in vivo detection as optical fiber can be inserted into human body.
Previous attempt for reflection setup for LSPR fiber-optic sensor [ 16] has utilized random gold nanoparticles produced by annealing thin gold film deposited on the end face of a multimode optical fiber, which has poor uniformity and reproducibility.
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For the experiments described in this work, we used a prism type total internal reflection (TIR) setup in order to maximize the useable field of view.
Photobleaching in the presence of an oxygen scavenging system (5% β- d-glucose, 10 mM β-mercaptoethanol, and 1% GODCAT solution, a mixture of glucose oxidase and catalase) was carried out with a single molecule total internal reflection microscopy setup excited by a laser beam.
The microarrays were imaged by combining a total internal reflection fluorescence (TIRF) setup with the SPAD imager (Fig. 1 ).
With this setup, a reflection spectrum of the nanofluidic sensor was obtained at fixed wavelength 637 nm while tuning the incident angle of laser source.
The biomimetic platform can be combined with a Total Internal Reflection Fluorescence (TIRF) microscopy setup, which allows for the convenient observation of the time evolution of the gradient and the interaction of ligands with the lipid bilayer.
The total internal reflection fluorescence (TIRF) microscopy setup was described in [24].
We further investigated the angle-dependence of the blue reflection using the ARM setup, consisting of two optical fibers that can be rotated around the sample (see Methods).
The gray-tone mask design, reflection-type projection photolithography setup, enzyme etching processing, and the characterization of fabricated 11 × 11 gelatin microlens array, are presented in this paper.
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