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Although the enormous volume of data generated by genome sequencing projects, expressed sequence tags (ESTs), and cDNA analyses has improved our understanding of many processes, they often fail to reflect the influence of posttranscriptional modifications and protein interactions or offer a true reflection of protein levels or activity.
However, the 2D-PAGE appretainsetanns advantageage with regard to protein quantification since protein spot intensities and sizes on 2D-PAGE gels are a better reflection of protein abundance compared to abundances inferred from peptide MS data alone.
The mRNA amount is not a direct reflection of protein expression since post-transcriptional processes are significant to the final synthesis of proteins.
Instead it is more a reflection of protein degradation with subsequent accumulation of aromatic amino acids, whose aromatic ring is energetically "expensive" or unfavourable to break open.
An evident limitation of our experiment is that mRNA concentrations are not necessarily a faithful reflection of protein levels or activities.
Furthermore, even if serum and plasma levels of osteopontin were highly correlated with asbestos exposure [ 107], osteopontin can be hydrolyzed by thrombin and so the results might not be a true reflection of protein levels in the blood.
Similar(52)
To be able to determine whether the differences in SAA4 actually is a reflection of higher protein concentrations we used the samples with more equal protein concentrations from both groups and performed statistical calculations on this more homogenous set of samples.
However, the diminished fluorescence was likely a reflection of decreased protein stability because the T62I mutant did not accumulate appreciable amounts of GFP protein.
Such a fusion protein has been used in human cells, and appears to give an accurate reflection of total protein localization[42].
This could be a reflection of increased protein stability.
An alternative explanation for this correlation is that the increased VEGF is simply the reflection of increased protein leakage in the lung.
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CEO of Professional Science Editing for Scientists @ prosciediting.com