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GFP, which can be qualitatively and quantitatively measured by flow cytometry, was used as a surrogate in the study as an objective reflection of cells containing the transgene.
From these experiments, it was unclear whether centrosome disruption was a reflection of cells entering senescence, or whether these changes contributed to the fate of the cells entering senescence.
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Plaque development by FMDV on BHK 21 cell monolayers is a reflection of cell detachment as a manifestation of virus infection.
This may be a reflection of cell line-specific characteristics or due to the over-expression of IκBα upon reconstitution in these cell lines (G.Ghosh, data not shown).
Spectral variance in the FTIR data (Figure 3E) within the polysaccharide fingerprint region was difficult to assign to a single cell wall polymer and was more likely a reflection of cell wall reorganization.
Injured cortical areas, i.e. unstained tissue as a reflection of cell loss (Figure 2B), were measured using the computer image analysis system (Scion, NIH software, Bethesda, USA) and the distance between respective coronal sections were used to calculate a linear integration for the lesion volume determination as previously described [42].
Survivin expression was originally considered a reflection of cell proliferation.
The expression of versican by tumor cells appears to relate to higher mitotic activity and a reflection of cell proliferation [ 34].
It is possible that the local changes in acidity are not merely a reflection of cell kill, but may be part of the process.
For example, DNA synthesis activity may be quantitatively assessed using [F]fluoro-deoxy L-thymidine (FLT), as a reflection of cell proliferation and tumour growth [ 31].
In this respect, although Ki67 is a primary marker of proliferation, it can also be a secondary reflection of cell death (Archer et al, 2003).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com