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Furthermore, a proper experimental setup has been made, based on a reflection microscope, for direct measurements of the crack tip displacement field by the DIC technique.
Equiping a total internal reflection microscope with a newly designed wedge prism, we obtained a spectral image of a single rhodamine red molecule attached to an essential light chain of myosin.
Before loading the sample, we checked the homogeneity of the sample with a grain size less than 2 μm again by using a high-resolution reflection microscope and selected only homogeneous pieces of the mixture foil.
Gliding of the microtubules was observed total internal reflection microscope and their velocity was measured using the manufacturers' software (Olympus Biosystems GmbH, Planegg, Germany).
To simultaneously trap and image microparticles, optical tweezer systems are usually built in conjunction with epifluorescent and TIRF (total internal reflection) microscope systems [24].
Measurement: A total internal reflection microscope (TIRM) system is used to measure the scattering light.
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Single-molecule measurements of probe-target hybridization rates and T7 RNAP transcription were performed using a home-built multi-color total-internal-reflection microscope as described (Revyakin et al., 2012).
The surface appearance and microstructure were examined under a reflection optical microscope and a scanning electron microscope equipped with an in-situ energy dispersive X-ray spectroscopy, which can evaluate the elemental analysis of deposits.
Single-molecule FRET images were visualized by a total internal reflection fluorescence microscope equipped on an inverted type microscope (IX70 or IX71; Olympus), as previously described (Funatsu et al, 1995; Taguchi et al, 2001; Mori et al, 2007).
A prism-type total internal reflection fluorescence microscope was built using a Nikon Ti-E Eclipse inverted fluorescence microscope equipped with a 60× 1.20 N.A. Plan Apo water objective (Nikon Instruments, Melville, NY).
Total internal reflection fluorescence microscope analysis showed cells with numerous filopodia spread on D-2HVP-functionalized surfaces.
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