C > T, G > A transitions were frequent followed by transitions, A > G; T > C. The lack of SNP clustering coupled with the substitution biases may reflect Taq polymerase errors, and signal possible consequences of base mis-incorporation [ 40].
Krupovic M, Bamford DH: Does the evolution of viral polymerases reflect the origin and evolution of viruses?
Because our analyses used replicate PCR reactions of starting genomic DNA, high fidelity polymerases, plus sequencing and restriction mapping of total PCR products, we can exclude that the polymorphisms reflect rare in vitro polymerase errors captured by molecular cloning.
In addition to the bands corresponding to the allele sizes, the migration profiles of the CAn microsatellite markers contain extra bands that reflect slippage of Taq polymerase during PCR (-2 bp bands) and non-templated addition of an A residue (+1 bp bands).
A similarly analyzed RNA Polymerase II profile showed a more diffuse and tailing distribution, which might reflect the progression of the polymerase along transcribed genes.
Following the formation of the very initial polymerase RNA complex, additional steps were monitored that occurred independently of the RNA concentration and RNA moiety and most likely reflect conformational transitions of the polymerase.
The diverse phenotypic effects of dms4 mutations may thus reflect interactions with multiple RNA polymerases (He et al. 2009; Kanno et al. 2010).
We expected that the mutational events observed in HCT116 cells would reflect errors generated by DNA polymerases, which we have shown here and previously to be biased toward deletions.
Thus, regions enriched in GRO-seq reads reflect regions enriched in transcriptionally engaged polymerase.
"KAPA3G" DNA Polymerase was derived from a previously engineered, more processive variant of Taq DNA polymerase (processivity reflects the average number of nucleotides added by a DNA polymerase per association/dissociation event with the template; processive enzymes synthesize DNA more quickly and are more efficient in the presence of inhibitors).
