The fluorescence intensity reflecting bound antibodies was read by an Attune flow cytometer (Life Technologies, Carlsbad, CA, USA), gating on the bacteria with combined side scatter/Hoechst 34580 fluorescence.
She also wrote about the difficulty of stepping back into the world of 1847, when the novel was published: A novelist, we reflect, is bound to build up his structure with much very perishable material which begins by lending it reality and ends by cumbering it with rubbish.
The results showed less than 20 ng/ml APC in these cases (data not shown), indicating that findings during rhAPC treatment reflect APC bound to circulating MP-associated EPCR.
The tally was seventeen hundred and twenty-five delegates for Trump, four hundred and eighty-four for Cruz, a hundred and twenty-five for Kasich, a hundred and twenty-three for Marco Rubio, and single digits for a few others, reflecting delegates bound early in the process.
The idea was that an awareness function reflects some bound on the resources we can bring to bear.
Upon hitting a membrane surface, trajectories were reflected (or bound on transporters).
Concentrations administered reflected hydrazone bound DOX content in each micelle and was confirmed using spectrophotometer measurements in MeOH.
Our conservative target list reflects genes bound by Dichaete in independent binding assays and that show very significant expression changes in Dichaete mutant embryos.
Since Pab1 promotes Pan2 activity, one model is that this deadenylation reflects Pab1 bound to the first ∼65 residues of the A tail, but the 3′ most region is exposed and thereby rapidly deadenylated by Pan2.
"Once people sit down and reflect, there's bound to be a surge," Mr. DiClemente said.
