The situation described by the reviewer, in which a strand scission event occurs 5´ of the tethered radical source and reverse transcription starting at that position terminates at a second oxidatively damaged site, should still reflect a 'true' constraint similar to constraints produced by strand scission and reverse transcription termination both occurring 3´ of the tethered radical source.
Thus, we suggest that the widespread occurrence of tapered whiskers does not reflect a fundamental constraint on follicle development or physiology.
Considering that dipteran and hymenopteran insects diverged over 300 million years ago, the conservation of this microsynteny may reflect a selective constraint.
TRIM15 similarly plays a role regulating innate immune signaling, and its positive selection may reflect a similar constraint as TRIM25.
The promoter-sharing between CPNE1 and RBM12 and the conservation of this phenomenon during evolutionary courses probably reflect a selection constraint to keep the two genes co-regulated, which in turn suggest of a functional relationship between these two genes.
The importance of Eq. (57) is, therefore, not in its explicit form, but that it may reflect a structural constraint inherent in the connectivity patterns and the dynamics of cortical neuron populations.
The role of transposition in the evolution of tubulin repertoire may itself reflect a ubiquitous constraint in kinetoplastids, and the reason why tubulin tandem arrays exist at all, the absence of individual gene promoters.
This conservation of motif position within each subclass is consistent with the existence of a common ancestral gene for the Fox genes comprising an individual subclass [ 17], but may also reflect a functional constraint that maintains the position of the eh1 motif.
The reason is still unknown but may reflect an evolutionary constraint that primordial proteins consisted of a small set of primitive amino acids and gradually acquired new amino acids in the course of neutral evolution.
This may reflect an evolutionary constraint acting against gene loss and gain, such as for members of protein complexes where duplications may affect the stoichiometry of complexes [ 35].
