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These observations are primarily based on solution studies of various monomeric species and reflect a backbone-mediated effect of the PPII-structured PRD on the conformational ensemble of the nonaggregated polyQ domain.
The high conservation of these residues in the CHHC Zn-finger domains may reflect a requirement for a specific backbone conformation.
Such a linear chain may reflect the backbone of a canonical pathway such as a KEGG [ 33] pathway, but it may also reflect pathway crosstalk.
Recommendation #6: GROW A BACKBONE.
The backbone RMSD values for kinesin, however, were much greater in the no-nucleotide trajectory, reflecting a significant conformational rearrangement.
This likely reflects a combination of greater nuclease resistance of the DNA oligonucleotides and the tendency for nonspecific binding of the phosphorothioate backbone ODNs to the tissue.
She reflects a bit.
Although 41 backbone amide {H,N}-HSQC signals monitored during a W7 titration could be fit to a single-site binding model, numerous side chain methyl {H,C}-HSQC signals reflected a secondary binding process (9).
Finally, on a personal note, I think chemical probing with DMS (revealing the accessibility of the Watson-Crick positions) would add very convincing evidence to the Shape data that reflect only the backbone.
The significant deviations in the spectral shapes in the amide I region (1700 1600 cm–1) between the wild type and H166L, which reflect the protein backbone conformational changes, indicate differences in conformational changes between the two proteins.
Continuous wave EPR spectra from nitroxide labels that are engineered into proteins contain information on fast motions in the 0.1 to 100 ns time scale, and the spectra are known to reflect secondary structure, backbone motion and tertiary contact of the label.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com