To add, orient and refine the order of superscaffolds into an AGP for constructing chromosome-scale PMs, a separate process was implemented, as described below.
Conventional molecular marker maps (Supplementary Data Set 2) and syntenic inferences were then used to refine the ordering of scaffolds within the initial genetic bins.
Based on a high-density (∼21 K) SNP linkage map of rapeseed, Harper et al. refined the order and orientation of the A and C genome sequence scaffolds, constructed pseudomolecules representative of the 19 chromosomes of B. napus and successfully carried out associative transcriptomics of glucosinolate and erucic acid content in a population of 53 B. napus lines.
The processing of stereo DTMs followed the workflow described in Section 3. The topographic products were constructed and refined in the order of MOLA, HRSC, CTX and HiRISE data.
For iteration three, heterozygous regions were refined where the order of bins within heterozygous regions had changed after iteration two.
Stimulus parameters were successively refined in the order of orientation, spatial frequency, temporal frequency and contrast, based on on-line analysis of the responses of the target unit.
Since Orc6 appears to contact the subunit at the upper left end in the EM class averages, the Orc3 Orc6 interaction allowed us to refine the subunit order of the core AAA+ subunits to Orc1-Orc4-Orc5-Orc2-Orc3 fORCDrORCphila ORC.
As observed from Table 1, the contraction factors of errors (frac{|nabla u-u_{h})|_{0}}{|nabla u|_{0}}), (frac{|nabla u-u_{h})| _{0}}{|nabla T|_{0}}), and (frac{|p-p_{h}|_{0}}{|p|_{0}}) become smaller and smaller as the mesh is refined, and the orders of these relative errors are arou|_{(frac{1}{4}) as the mesh is refined once.
In practice, he accepted that most refined of honours, the Order of the Companions of Honour, from no lesser personage than Her Majesty the Queen.
Although refining could consume on the order of 200 KWH per BDt of pulp, the reduction in enzyme dose is a good economic tradeoff; if an existing mill did not have oxygen delignification originally, we find it is financially justified to build a new system.
