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A tool for refining the alignment results, MatchedIonsFinder [ 1], is also available via KOMICS.
Using ∼12,000 particles, we obtained a 3D map by refining the alignment of the two AAA+ rings only, but then including the whole molecule in the reconstruction, which was at ∼17 Å resolution.
After refining the alignment based on the secondary structures of ITS-1 and ITS-2 [ 10, 48] and reducing identical sequences to a single OTU, the alignment (676 positions and five OTUs; study ID: S15331 available from TreeBASE) were subjected to MP and ML (based on HKY85 model) methods based on a branch-and-bound search by PAUP 4.0 with bootstrap analysis based on 1,000 replicates.
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Figures 6 cd) show the results of refining the alignments from Figures 6 ab).
Our framework scans the initial mapping file from a gapped NGS aligner and refines the alignment of the soft-clipped reads meeting tiered criteria.
In summary, our method takes advantage of (i) second-order Gaussian derivatives detectors for localizing the markers [21], (ii) a cluster analysis to identify them throughout a series and (iii) iterative procedures to refine the alignment process.
Therefore, we refined the alignment of the point clouds at different times by means of cloud-based ICP registration based on features that are thought to stay in the same location, and do not change shape, distributed around the target zone.
Nerve derived signals disperse prepatterned clusters that are not contacted by the nerve, and thus refine the alignment between the pre- and postsynaptic apparatus [6], [7], [8].
Importantly, MUSCLE refines the alignment with a multitude of alternative guide trees and is not restricted to the guide tree chosen for progressive anchoring.
In the next step we refine the alignment between homologues in PALI database, obtained through a recognized rigid body method, using match of protein blocks in the local structurally variable regions.
MatchedIonsFinder was used to refine the alignment results.
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