The best two data sets in terms of resolution (wild-type ParC55) and completeness (Cys426 mutant ParC55) were used for the structure solution and refinement (Table 1).
The water molecules, acetate molecules and phenylalanine molecules were added manually where Fo-Fc electron density at ≥3.0 σ contour level and justified by hydrogen bonds or hydrophobic interactions in the final stages of refinement (Table 1).
The CC 1/2 value improved significantly with post-refinement (Table 3).
Neither site was completely phosphorylated, so occupancies of the two sites were estimated during refinement (Table I).
The first part consists of six core values: freedom, fairness, wellbeing, replacement, reduction, and refinement (Table 1).
The R-factor and average overall Fourier shell correlation were monitored during refinement (Table 1) and the final model was validated using MolProbity (Chen et al., 2010).
Mucinous adenocarcinoma was assigned as 3 points, signet ring cell carcinoma was assigned as 4 points, undifferentiated carcinoma was assigned as 4 points, a special type of stomach cancer as 4 points (these score still needs further refinement) (Table 1 ).
Both structures were then completed using iterative cycles of model building and refinement (Table 2).> Like T. congolense HpHbR, the T. brucei receptor is elongated, consisting primarily of a three-helical bundle: helix I (red; residues 42 110), helix II (orange; residues 116 182), and helix V (dark blue; residues 224 296) with a total length of 118 Å.
Crystallographic data and refinement statistics are shown in data collection and refinement statistics table.
To assess the side information quality improvement during the different refinement passes, Table 3 reports the PSNR values of the interpolated frames for the two sets for GOP values of 2, 4, and 8.
