Refinement of tissue-level prolactin and prolactin receptor expression assays may provide a more accurate estimate of the prolactin levels surrounding breast epithelial cells from autocrine and endocrine sources.
Ultimately, what will be required to answer these questions thoroughly are greater genetic and physical resources, additional genetic variation that can be used to distinguish with confidence chimeric cells and a refinement of the tissues and cell types that can be studied.
Continuous refinement and improvement of tissue engineering strategie is seen, but a number of tough practical problems persist, including the scarcity of tissue biopsy material and the difficulty in cell expansion maintaining the phenotype.
With a growing number of lncRNAs implicated in transcriptional gene regulation, this view may need refinement to include networks of tissue and developmental-stage specific lncRNAs that complement known regulators to tightly control gene expression and thereby organism complexity [ 12, 13].
Other refinements in tissue engineering include the use of stem cells, cell pre-selection and growth factor pre-treatment of cells that are used for seeding scaffolds.
In fact, in the IDP model, the development main priority is efficient use and redevelopment of existing tissues and refinement of urban land uses(Table 5).
In fact, in the IDP model, the development main priority is efficient use and redevelopment of existing tissues and refinement of urban land uses.
In terms of improving differential response between tumour and normal tissues, further refinement of dose fractionation patterns would be needed, but also research should continue on the modification of response using drug/radiation protocols, targeting techniques, growth factors and other biological response modifiers to support normal tissues, and modulation of DNA repair.
Ongoing acquisition of pre-treatment tissue will allow refinement of histone mutation-based risk groups and molecular signatures, which have potential to elucidate oncogenic mechanisms and resistance pathways.
In images before and after deformable registration and manual refinement, the SSD provides an indirect measure of the registration quality as the position of tissue changes.
