However, a role of miRNAs in the formation of the MHB and in the refinement of the Wnt1 expression pattern at this boundary has not been reported so far.
We identified and characterized several enhancer-trap Flp lines for use in larval imaginal discs, and the results of our experiments show that FINGR is a powerful method that enables refinement of the GAL4 expression patterns within the existing collection of GAL4-UAS tools in Drosophila.
First, many experimental strategies, including the refinement of expression patterns using intersectional genetic approaches, require independence between the transcriptional control of multiple genetic components (Luan and White 2007; Simpson 2009).
Because the secreted Wnt1 protein plays a prominent role in isthmic organizer function, we focused in particular on the refinement of Wnt1 gene expression in this context.
Analysis of the data from the three month insulin intervention experiment in the context of this one month duration of diabetes experiment (i.e., prior to initiation of insulin treatment) permits further refinement of gene expression patterns into more specific Rescued and Prevented categories.
In order to have the most stringent refinement of the network we integrated the gene expression data with the MEN [ 33, 37, 38], and then the Dapple algorithm [ 42] was again employed to rank the gene expression loci that aligned to the MEN, to provide a further level of statistical robustness.
Further analysis of our gene expression data, including refinement of the signature associated with oAC treatment, will be published elsewhere.
Along with a preoccupation in literature with death and damnation, there appeared a contrasting refinement of expression and sentiment bred of nostalgia for the courtly, chivalric ideal.
Perhaps the protected location is a failsafe mechanism to ensure refinement of expression to single progenitors.
These are becoming increasingly useful in the refinement of the gene structures, especially in genes with a low level of expression that often lack transcript data.
