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Refinement of the approach, combined with site-directed mutagenesis experiments, yielded optimized consensus phytases with Tm values of up to 90.4°C.
This shows promising to further development and refinement of the approach by expanding it with other potential factors to eventually build a robust user differentiation subsystem.
Future studies are necessarily applying IM Adapt to a variety of EBIs and public health issues to allow for continued evaluation and refinement of the approach.
In a refinement of the approach, termed asymmetric hybridization, the donor cells are irradiated prior to the fusion process, which has the effect of reducing the contribution of the donor's genome to the somatic hybrid, thereby promoting the introgression of smaller donor segments [ 4, 5].
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For the purposes of risk-of-bias assessment and for assessment of treatment effect, we identified for each trial a single 'main outcome measure' using a refinement of the approaches adopted by Linde et al. [ 10] and by Shang et al. [ 11].
Refinements of the approach have led to the incorporation of further markers including stromal response markers and microvessel density [ 32].
Both bounds B1 and B2 are derived by enumerating HGT scores rather than enumerating permutations in S N. The refinement of this approach produced by reducing the extent of multiple counting of permutation further improves the upper bound.
A possible refinement of this approach is the inclusion of probability distributions for the anticipated positional changes [ 13, 14].
This systematic review is the first step in consolidating this information to assist in the refinement of this approach.
Referring to the first research question, the inclusion of environmental data constitutes a meaningful refinement of the assessment approach.
Recommendations for the refinement of the methodological approach are included for future global or scaled regional risk assessments.
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