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Animal suffering avoidance and refinement of procedures are included in CEA aims.
Refinement of procedures to improve the welfare of the animals, such as use of analgesics, avoiding significant adverse effects, and enhanced housing conditions, were adopted.
The documentation obtained from this early experience permitted refinement of procedures and development of a user manual.
This is an interesting finding and, if the mechanisms were better understood, refinement of procedures that target these mechanisms in treatment may lead to improved outcomes.
The case study method allows researchers to be guided by what is observed in the field; it also provides new facts that can inform assumptions, hypotheses, and further refinement of procedures (15).
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Over time, survival of patients undergoing HCT has improved with serial refinements of procedures, donor selection and toxicity management.
Furthermore, the results of this study highlight that further refinement of standardization procedures in PubChem will be beneficial.
Set-up involved preparatory work, refinement of logistics procedures, and training.
Further refinement of isolation procedures and proteomic and lipidomic analysis will provide better insight into composition under various nutritional conditions (Connerth et al. 2009; Grillitsch et al. 2011).
We relate our analysis only to the currently annotated Ensembl reading frames, although these are in a constant flux, due to curation and further refinement of annotation procedures.
The possibility to follow the same animal from the beginning to the end of treatment and to analyze more parameters at ones, can allow better statistically evaluation of data and underlining the concept that strong efforts should be dedicated to the refinement of experimental procedures that limit the number of animals needed for experimental models of human disease.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com