A precedent setting legislative mandate under the Canadian Environmental Protection Act 1999 to establish priorities for assessment based on systematic consideration of all of the approximately 23,000 Existing Chemicals in Canada required the development and refinement of methodology in a number of important areas.
This proposed view of how to consider gene expression (or similarly peptide/metabolite abundance) in a genetical context is currently receiving considerable attention, extension and refinement of methodology [ 21- 25].
FM contributed in various ways in the work reported in this paper including; refinement of methodology, data collection, data analysis, inputs to various versions of manuscript, editing and approval of final version.
The techniques involved (see Graphical Abstract) also provide platforms for research and refinement of breeding methodologies.
We share this information as part of our report aiming on refinement of the methodologies we used.
Refinement of the methodologies for processing serum samples, including initial depletion of high-abundance proteins, is likely to substantially increase the information that can be derived from the resulting profiles.
Average watershed values cannot take this situation into account and therefore our procedure is a step forward in the spatial refinement of LCIA methodologies (see further explanations in SI S9).
However, expansion and refinement of research methodology over the last decade, with more researchers replicating previous studies, may make broader application of research findings possible.
The process of making mathematical logic rigorous and of demonstrating the usefulness of the techniques in pursuing mathematical ends that was pursued so successfully in the first half of the twentieth century represents a coherent refinement of logical methodology.
Further investigation and refinement of the methodology to classify and verify isoform sub-clusters is needed, particularly to extend the technique to more distantly related species.
