Exact(6)
Three types of amino acid representation in the electron density are examined in detail: (i) sharp with very clearly resolved features; (ii) well resolved but clearly divided into two conformations which are well behaved in the refinement, both having high quality geometry; (iii) poor density and difficult or impossible to model.
Since then, there has been refinement both in terms of patient selection and supportive care.
The subsequent refinement consisted of alternating rounds of positional minimization, individual restrained thermal factor refinement, both using the MLF maximum likelihood target function, and model building.
Prior to 3D refinement, both data sets were subjected to reference-free 2D class averaging and 3D classification to identify structurally homogeneous subsets.
After refinement both peripheral metal ions had similar B-factors to their protein ligands, and the Fo(EDTA1) − Fc(EDTA1) electron density map no longer showed strong difference features, indicating sodium is likely to be bound in both sites.
Care was taken to implement the 3 Rs rule (replacement, reduction, and refinement) both to reduce the number of animals used and the suffering during the experiments.
Similar(54)
Thus, post-refinement both improves diffraction data quality for a given set of images and reduces the number of diffraction images required for structure determination and refinement from serial diffraction data.
But Barber's colleague Ned Rorem, in a perceptive article from 1982, challenged much of this as "conventional wisdom," commenting on the "unpredictable" and "neurotic" qualities, as well as the essential refinement, of both the music and the man: "Is elegance indeed the term for the person, and is his product inevitably suave?
An interesting refinement of both inequalities in (1.1) was obtained by Raïssouli and Dragomir in [10].
Then α is a lacunary refinement of both the lacunary sequences β and θ.
Moreover, the current framework can be applied for refinement in both random and physical space.
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