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Models of SUR1 ABC transporter module were refined against the focused refined map in respective states.
Specifically, models of Kir6.2 transmembrane domain and SUR1 TMD0 were refined against the focused refined map of transmembrane domain in the ATP + GBM state.
Models of Kir6.2 CTD were refined against maps after focused classification and refinement of Kir6.2 CTD in the ATP + GBM state.
The particles in this class were refined against the class map in RELION and refinement converged after 16 iterations.
The model was then refined against the native data set to 2.35 Å resolution.
These initial structures are refined against the observed data and are used to generate initial phases.
We obtained a Calpha backbone model for each subunit from an initial homology model refined against the cryo-EM density.
The model of full-length GG-SpaD was refined against the anisotropically truncated native data (Table 1).
The three-dimensional crystal structure of isotactic polypropylene in its β-polymorphic form has been refined against electron diffraction intensity data.
The apo ag85B model is refined against 1.8 Å data, to an R-factor of 0.196 (Rfree is 0.276), and includes all residues except the N-terminal Phe.
The r.m.s.d. between the model refined against half-map and compared to the full map, and the one refined against the full map is 0.984 Å, and their corresponding FSCs against the final map show a resolution difference at the 0.5-cutoff of approximately 0.1 Å.
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