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The first component of our toolkit are, of course the software tools used to drive the diffractometers, process the captured X-rays, solve and refine the structures and then display and interpret the results.
This method uses the miRNA-target predictions as the basic structures, while the expression profiles of miRNA and mRNA are applied on the structures to find the co-expressed miRNAs and mRNAs, then PPIs are further used to refine the structures.
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The products are studied by XRD to refine the structure.
Certainly more restraints would enable a researcher to better refine the structure and to discover discrepancy within the restraints.
Furthermore, RNA-seq can detect genes expressed at low levels and refine the structure of transcripts (Wang et al. [2009] Wilhelmm and Landry [2009]).
The SAR analyses will guide us to further refine the structure of the thieno[3,2-d]pyrimidine derivatives to achieve optimum anticancer activity.
To refine the structure and content of chromosome 22q11.2 LCRs, we applied computational analysis to dissect region-specific LCRs using publicly available sequences.
The templated structure was then subjected to 50 nsec of dynamics without constraints to refine the structure.
With further NMR studies on the horizon we hope to further refine the structure using a combination of computational and experimental approaches.
Synchrotron X-ray diffraction data allowed us to solve and refine the structure of the complex at 1.45-Å resolution (Table 1), much higher than previously obtained for the CaM-CnA-CBD complex (between 1.86 and 2.6 Å).
Our results refine the structure of the Math5 transcription unit, explore the concept of an intronless gene, and provide a cautionary lesson for PCR-based studies of RNA processing.
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