These findings suggest that G-CIMP defines a subset of proneural GBM and can be utilised to further refine expression-defined subtypes.
To refine this expression signature, we interrogated patient time course data for genes whose expression was specifically upregulated in AR patients as they approached rejection using an artificially defined pattern of increasing expression.
We found that Emx2 antisense transcripts may both stimulate and refine Emx2 expression.
Different classes of small RNAs (sRNAs) refine the expression of numerous genes in higher eukaryotes by directing protein partners to complementary nucleic acids, where they mediate gene silencing.
miRNAs refine gene expression post-transcriptionally either via the cleavage of target mRNAs or the inhibition of translation of target mRNAs depending upon complementarity between miRNA and the 3' untranslated regions (UTRs) of targeted mRNA in animals and plants [4] [8].
In this work, we leveraged the CV analysis of biological replicates to refine our expression measurements and to correct systematic biases associated with GC-rich genomes.
MicroRNAs (miRNAs) are endogenous, small, non-coding RNAs of 18 25 nucleotides that regulate and refine gene expression at both transcriptional and translational levels.
It should be emphasized, however, that for many other GPCRs such as BAI2, BAI3, CD97, GPR111 and GPR112, our results confirm and refine the expression patterns found by the aforementioned group.
