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Most of the conversions predicted by the models were observed (high specificity) but a substantial number of conversions were not predicted (low sensitivity), indicating that the models could be refined by integrating additional information.
Such lengthy lists can be partially refined by integrating spatial and temporal expression data for both miRNAs and their targets.
With the new findings provided by NGS, the cytogenetic model was proposed to be refined by integrating the analysis of recurrent gene mutations, since most of them demonstrated to have a clinical independent impact on patient survival.
Future studies should also be conducted to refine the model by integrating other sources of data (e.g. additional medical claims, pharmacy and hospitalization data).
Coupling the GPR with a metabolic model is a starting point for several algorithms designed to refine metabolic models by integrating high-throughput gene or protein expression data.
We further refined the model by integrating tissue specific information to better represent the distribution of metabolism to root and shoot of the plant.
Coronello and Benos [ 31] refined the combinatorial model by integrating miRNA expression levels in the scoring function.
The final step in the model refines question (2) model by integrating significant factors from questions (1a) and (1b) as covariates (in this case, questions (3a) and (3b)).
In [ 36], the authors improved this method by employing a probabilistically constrained combined graph search-graph cut method refines the candidate SEADs by integrating the candidate volumes into the graph cut cost function as probability constraints.
A first step would be to refine the current in vivo strategies by integrating information derived from in vitro and nonmammalian alternative test strategies.
Hans Kristian Moen Vollan and Carlos Caldas [ 12] discuss how results of next generation sequencing hold the potential to refine molecular classification of breast cancer by integrating information from genomic signatures to existing knowledge derived from histopathological sub-classification.
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