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After MEGABLAST identified the sequences, we refined alignments with the bl2seq tool with a word size of seven and filtering disabled and with manual refinements.
Reprojections of the averaged model were used to refine alignment of all selected segment images.
Importantly, MUSCLE refines the alignment with a multitude of alternative guide trees and is not restricted to the guide tree chosen for progressive anchoring.
The locations of 13 protein-coding genes and two rRNAs were initially identified by DOGMA [ 85] with default settings, and refined by alignment with mitochondrial genomes of Panulirus japonicus (NC_004251) and Squilla mantis (NC_006081).
Sequences of tRNAs, rRNAs, and noncoding loci were first aligned with the default parameters, then realigned with the refining alignment option.
Refined alignment and sequence to structure mapping was performed in Insight II and with Slide, a interactive threading software [38].
The manually refined alignment is shown in the Supporting Information.
The exon-intron boundaries were refined manually by alignment with Hox sequences of other vertebrates.
Sequence sets (protein or nucleotide) were aligned with Muscle [ 41], and manually refined (alignments are available upon request).
ER then refined the themes in consideration of their alignment with the existing literature.
The alignment with LmjF was done mainly to refine the analyses of reads mapping onto tRNAs.
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CEO of Professional Science Editing for Scientists @ prosciediting.com