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*: refers to the effect of wild type EDM2 or WNK8 on expression of the respective gene set **: refers to transcript levels of individual genes in the indicated genotypes or presence of genes in defined gene sets: "AND" and "NOT" are used according to Boolean logic.
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As we know little of the stability of yir RNA at present the data presented refers to transcripts detected within the developmental stages analysed.
Thus, when we refer to transcripts in this study, we are referring to the representative transcripts chosen for each locus.
To ensure a contextualised analysis, we referred to transcripts as a whole as well as to particular data segments.
A unique RefSeq ID refers to each transcript.
"SOLiD ORA" refers to the transcripts determined from our experiment using ORA, "Tiling arrays" refers to the data reported by Xu and colleagues [ 10], "5'-RACE" and "Illumina sequencing" refers to data reported by Nagalakshmi and colleagues [ 9] and "SOLiD Cufflinks" refers to the transcripts reported in our experiments and analyzed using Cufflinks [ 20].
The 3'/5' signal ratio is designed to detect the 3' and 5' regions of the mRNA; the 3'/middle ratio refers to the mRNA transcript from 3' to the middle of the mRNA transcript.
The most common meaning of the term 'antisense transcript' refers to a protein coding sense transcript and a fully processed (capped, polyadenylated) antisense RNA with complementarity in exonic regions.
When describing expression patterns, a gene or an "ID" (shorthand for "identifier") therefore refers to a predicted transcript whose sequence guided the design of a Probe Set on the Lotus Affymetrix array.
With this information, we were able to identify a subset of probe set)s of specific genes that targeted a common set of transcripts across all 4 microarray platforms, which we refer as to "transcript pattern" (Fig. 1).
Annotated transcript properties refer to PlasmoDBv10.0 transcript models This visual approach also highlighted two distinct lncRNA expression profile deviations during RBC rupture and parasite invasion [purple arrows in Fig. 3B].
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