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The dataset for CASMI 2016 was predominantly well-known anthropogenic substances and as a result there are many distinct and highly referenced substances in the candidate lists.
C22 was selected as a reference substance.
Fig. 1 HPLC chromatograms of reference substance and test solution.
Therefore, using a reference substance for transformation studies seems necessary.
As a reference substance, 3,5-dichlorophenol was used.
Antipyrine was added to the maternal reservoir of all placentae as reference substance.
After the relevant research literature and reference substance comparison, Cp.23 was inferred as isopimpinellin.
To overcome this problem, a biodegradable standard reference substance should be included in each test series.
The test is considered valid, if the reference substance surpasses 60% degradation at the plateau phase.
Each batch included a control flask and five test or reference substance flasks.
As the test method is designed to assess particularly surfactants, we recommend using also a surfactant as the reference substance.
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