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Meeting abstracts were not included; however, references generated from published guidelines and reviews were also investigated.
A manual review of the references generated from the computer-search was also done.
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This build will include the API reference generated from Doxygen markup in the source tree.
Five replicates for each sample category were generated, and compared with a global reference, generated from an equimolar mix of amplified RNAs from each of the 15 plants.
For lung cancer cell lines, an unpaired, pooled reference generated from the intensities of all 45 normal lung tissue profiles (those matching the tumors described above) was used.
Additionally, our results are not reported in relation to C57BL/6 J (as both NGS and aCGH are), but as relative to a diploid reference generated from all strains.
Available literature references were generated from PubMed; the cross-references and identifiers of external databases were also imported.
If model bias was detected, new references were generated from common lines of the subsets until stable, model independent reconstructions were obtained.
Standard curves (cycle threshold plotted vs. log of concentration) for target genes and for the endogenous references were generated from a serially diluted cDNA pool of all tested samples.
All experiments were conducted following a reference design with the reference sample generated from a pool of RNA extracted from all sediment exposed worms as well as from the laboratory control.
The algorithm relies on general conservation of the ratio between actual ET and a reference ET, generated from satellite insolation data and standard meteorological fields from a mesoscale model.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com