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References for relative transcript levels were set to 1.
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The OsACT1 gene (forward primer: 5'-ATGAAGATCAAGGTGGTCGC-3'; reverse primer: 5'-GTACTCAGCCTTGGCAATCC-3') was selected as an internal reference for relative quantification.
Next, the experiment was repeated with a featureless, annular gap between the disk and the reference surface (Figure 4Aii), and finally, if the subject was still able to do the task, the disk was presented on a featureless gray background with only the edges of the display screen available as a reference for relative depth (Figure 4Aiii).
The laboratorial strain S288C was used as reference for relative genome profiling.
The reaction product served both as a control for RT PCR performance and as a reference for relative quantification.
VDAG_05595 was therefore used as a reference for relative gene expression analyses in accordance with Pfaffl et al. [ 58].
Cr is generally considered to be a stable metabolite; many studies apply Cr as reference for relative metabolite quantification.
The reaction product served as a control for RT-PCR and as a reference for relative quantification of hTERT mRNA and hTR.
RT PCR for the mRNA encoding the housekeeping gene PBGD was equally processed in a separate tube as a reference for relative quantification of hTERT mRNA expression.
An actin gene (Genbank accession number EB 136338, primers: 5′-GGCTGGATTTGCTGGTGATG-3′ and 5′-TGCTCACTATGCCGTGCTCA-3′) was used as the reference for Relative Quantification Analysis (RQA).
The validation set of mRNA samples were co-hybridized with a commercial RNA reference (Stratagene) supplemented with a routine set of tumor genes [ 83] to provide a standard reference for relative expression.
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