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Water table and outflow conditions for the remaining treatment plots and the reference were evaluated using several hydrologic criteria.
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The lag between the System sensor and YSI reference was evaluated using a model that characterizes delay with a time constant.
Quality of relevant studies identified through automated search and hand search of references was evaluated using the Newcastle Ottawa Quality Assessment Scale (Wells et al. 2011).
Using the developed rubric, a total pool of 1058 references was evaluated using the rubric with 88%100%% inter-rater reliability for each category of authenticity.
The expression stability of these candidate reference genes were evaluated using the four methods described below.
Levels of mRNA from HRG and 3 reference genes were evaluated using qRT-PCR.
Contigs that could not be aligned to the reference genome were evaluated using BLAST to identify plasmids.
Moreover, the significance of differences between the measured and reference volumes were evaluated using a two-tailed paired t-test.
All PCR experiments were repeated twice, and amplification efficiencies for both target and reference genes were evaluated using a standard curve over 1 5 serial dilutions.
Four reference genes were evaluated using a gene stability algorithm [ 51]; 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase (ATIC), glceraldehyde-3 phosphate dehydrogenase (GAPDH), mitochondrial ribosomal protein S7 (MRP S7) and mitochondrial ribosomal protein S25 (MRP S25).
The reference genes were evaluated using ER+ and ER- IBC, normal breast tissue and ER+ cell lines, and the data were analyzed using 1) descriptive statistics (i.e. boxplot and absolute value comparisons), 2) geNorm and 3) NormFinder programs that identified the most stably expressed genes.
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CEO of Professional Science Editing for Scientists @ prosciediting.com