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Each reference was verified manually to ensure the citation of valid interactions.
All pressure monitoring systems were calibrated in accordance with the manufacturer's specifications and the zero reference was verified before data collection.
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At last, the reference is verified by an experimental case.
Stable expression of reference genes was verified based on replicate samples (n = 4 from each group) with equal amounts of total RNA in each reaction analyzed using the 2-ΔCt method, and expression values normalized to the average Ct of three stable reference genes (CoxI, CyCTI-3, and Eif5a) using the ddCT method [ 52].
A leafy spurge SAND family gene was used as a reference; this gene was verified to be stably expressed during seed and bud development [ 38].
The model is described and characterized in details in reference [22], where it was verified that the cloned construct MOP-EGFP exhibits properties similar to the native MOP that the construct inserted in the plasma membrane, is sensitive and selective to its specific ligands and coupled to the cellular trafficking machinery (Fig. 1A).
The validity of the method was verified using reference objects of known geometry and the accuracy obtained was in excess of 98%.
After the interview information was verified by reference to antenatal and other medical records.
Also, the capability of HPRT gene as an internal reference gene for our model was verified (its expression is not affected by T. cruzi infection).
Correct intra-MePD placement of cannulae connected to the osmotic minipumps filled with GABA or glutamate antagonists was verified with reference to the atlas of Paxinos and Watson (39), and histological analysis is displayed in Figure 5.
Timely completion of the experience-sampling forms was verified with reference to (i) the reported time of form completion and (ii) the reported time interval between the actiwatch signal and form completion. Following standard guidelines (Delespaul, 1995), 11 participants who responded to less than one-third of the alarms within 15 min were excluded from the analysis.
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CEO of Professional Science Editing for Scientists @ prosciediting.com