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The ferric-reducing antioxidant power (FRAP) of the extracts and reference was tested using the assay of Oyaizu [16] based on the chemical reaction of Fe(III) = > Fe(II).
The ability of source leaf phloem to take up L-[1-C]AsA, or a number of substrates related to AsA metabolism ([1-C]DHA, D-[U-C]mannose, L-[1-C]galactose and [U-C]sucrose as a reference) was tested in leaf discs of Nicotiana benthamiana, a model system for phloem uptake studies as it allows good resolution of major and minor veins [ 28].
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Accordingly, three specimens one of which was the reference were tested in the laboratory.
Differences in expression between AID- or disease-specific loci and the whole Gencode reference were tested using the two-tailed Fisher's exact test, and the P-values were corrected for multiple testing with the Bonferroni correction.
The suitability of both Ubiquitin (BN00705) and Elongation Factor 1α (EC 959059) as gene references were tested on cDNA from the berry developmental series (data not shown).
Therefore, a combination of β-Actin (Syber Green external reference gene) and RXRβ (Taqman internal reference gene) was tested.
The expression stability of eight candidate reference genes was tested in leaves, roots and callus.
A certain concentration (about at the middle of linear range) solution of 11 reference compounds was tested.
The combination for each reference strain was tested in triplicates.
The efficiency of target and reference amplifications was tested to be approximately equal.
Variation 1: The reference procedure was tested as described in EN 1500.
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