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The reference was pooled normal male DNA.
The labeled reference was pooled (20 μL final volume).
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The two labeling reactions (test and reference) are pooled and purified using Amicon Ultra 0.5 columns.
Labeled cDNA from one experimental sample and the common reference were pooled together and hybridizations were performed at 42°C for ~ 16 hours as described in Booman et al. [ 21].
The common reference RNA was pooled from ten wild type B6/129 strain mouse livers.
The reference RNA was pooled before use for hybridization.
The reference RNA was pooled before it was used for hybridization.
The reference sample was pooled from all 8 samples (2 time-points of infected or non-infected plants; 2 biological replicates were performed).
A fraction of total RNA of each sample was pooled to create a common reference pool which was further processed in the same way as the rest of the RNA samples.
Tumor and reference DNA were pooled, mixed with 50 µg Cot-1 DNA (Invitrogen) and concentrated to a volume of 20 µl in a vacuum centrifuge.
Labeled sample and reference DNA were pooled with 125 μg C0t-1 DNA (Roche, 1581 074) and precipitated.
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