Exact(5)
The MATa VGIV strains (LA390 and LA392) reported herein were co-cultured with each reference tester strain.
However, the strains LA 390 and LA 392 failed to mate with the MATa reference tester strains B4546 [29] and JF109 [28] (data not shown).
The mating type was confirmed by mating experiments with the C. gattii, serotype C, MATα reference tester strain NIH312 [27] as well as with the MATα supermater strain JF101 [28] (Figure 2).
The fertility of MATa VGIV strains was investigated by mating them with the following MATα and MATa reference tester strains: C. gattii clinical, serotype C, strains NIH312 (MATα) [27] and B4546 (MATa) [29], and crg1α mutant derivatives ("supermater" tester strains), JF101 (MATα) and JF109 (MATa) [28].
To create an intraspecific cross using a reference design, one genotype was selected as a reference (Tester), simulated reads from the Tester strain were mixed with each of the remaining 94 genotypes (Lines).
Similar(55)
Briefly, ΔCT values were obtained and ΔΔCT values (fold differences between reference and testers) were calculated.
10 μg of both tester and reference total RNA was used per hybridisation.
The cDNA under investigation was designated tester, and the reference cDNA was designated driver.
Alignments were compared to determine which reads aligned better to the Tester or Line references and which reads aligned equally well to both the Tester and Line references.
Mr Tester makes frequent references to farm equipment.
The mixed sets of reads were independently aligned to the exon regions in the Tester or Line references using bowtie [-k1 -m1] [ 60] and LAST [-l 20] [ 61].
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