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A total of 196 viral isolates were available (133 carried the resistance mutation); of these, 113 (79 with the resistant genotype) were reference tested by the VIRGIL laboratory.
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We have also estimated the extent of occurrence of false-negatives in the reference test, by Discriminant Function Analysis, in order to correctly evaluate all diagnostic tests.
On the same day, all patients underwent reference testing by repeated compression ultrasonography of the symptomatic leg, performed with a real time, B mode, linear array sonographic scanner at 5.0-7.5 5.0-7.5steMHz GE/systemon).
In addition, our study incorporated several features that strengthen its internal validity – consecutive enrollment of a relevant population, clear description of included and excluded patients, blinded reading of index and reference tests by trained non-research staff, and a gold standard which incorporated multiple culture results and follow-up assessment [ 40, 41].
and stability of candidate reference genes tested by the macros GeNorm [ 31] and NormFinder [ 32].
In contrast, of the 10 commonly used reference genes tested by Zhang and colleagues in human neutrophils [ 5], TBP appeared to be the least stable.
The clinical C. albicans strains and C. albicans ATCC 10231 reference strain, tested by the Kirby-Bauer disk diffusion susceptibility test, did not exhibit the zone of inhibition of growth.
The modification is that anti-dsDNA antibody levels should be above laboratory reference range (or twice the reference range if tested by ELISA).
20, 21 In this study, there were differences in those FNBs that were verified by the reference tests compared with those that were not.
Differences in the frequency of high or low results based on reference values were tested by Pearson chi-square statistics.
Total sperm count distribution differences between the Faroese men and the WHO reference distribution were tested by χ analyses.
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