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To verify the results, replicate calibration curves were compared to the reference substances for three consecutive days.
Apigenin-6,8-di-C-β-d-glucoside, isoviolanthin and apigenin-6-C-β-d-xyloside-8-C-β-d-Glucoside were isolated from the leaves of D. officinale via preparative liquid chromatography as reference substances for the experiments.
In order to compare the skin permeability of X. laevis to mammalian skin data, caffeine and testosterone were chosen as test compounds since they are well known as reference substances for performance with mammalian skin [12].
It also provides more than 2900 reference substances for national drugs, biological products and medical devices.
Adducts are now available as reference substances for developing sensitive detection methods.
BHT (2,6-di tert-butyl-4-methylphenol) (2,500 μg/mL) and ascorbic acid (2,500 μg/mL) were used as reference substances for free radical scavenging activity (positive control).
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Therefore, using a reference substance for transformation studies seems necessary.
Zinc seems to be a suitable reference substance for monitoring the sensitivity in G. tigrina tests within only 24 h of exposure.
EIMS spectra were recorded on a Finnigan MAT 95 spectrometer (70 eV) with perfluorokerosine as reference substance for HR-ESI-TOF-MS (Japan).
1,1-Dimethylbiguanide hydrochloride and fenformin hydrochloride (Sigma-Aldrich, St . Louis MO) were used as a reference substance for metformin and internal standard in the assay, respectively.
A 1,500 mg bolus of iohexol, a substance that is almost 100% filtered at the glomerulus and unlike creatinine is not secreted in the kidney tubules, thus representing the reference substance for the real change in GFR in humans, was injected intravenously on days 0, 7, and 14 (follow-up) and the iohexol plasma concentration was measured.
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