Sentence examples for reference level of expression from inspiring English sources

Additionally, we performed RNA-seq on a pool of epidermal melanocytes to determine a reference level of expression for each gene in normal melanocytic cells (see Materials and Methods).

For each sample, a no-reverse transcription control was used, and HPRT was used as reference level of expression.

The dashed red line represents the iBAQ value of genes that are not targeted by any miRNAs identified in this study and serves as a background, reference level of protein expression.

To quantify tissue specificity, we applied a binary score to expression in each tissue without reference to level of expression.

The appropriate application of quantitative real-time PCR (RT-qPCR), however, requires the use of reference genes whose level of expression is not affected by the test, by general physiological conditions or by inter-individual variability.

Although the community-accumulated tool set for the molecular analysis of Neurospora is extensive, two components are missing: (1) dependable reference genes whose level of expression are relatively constant across light/dark cycles and as a function of time of day and (2) a catalog of primers specifically designed for real-time PCR (RT-PCR).

A subset of more highly expressed rye reference sequences with a minimum level of expression of at least 10 transcripts in any rye tissue-specific library and not detected in all triticale libraries were selected for further analysis.

However, there were concerns for the use of 18S gene as a reference, as its much greater level of expression relative to target mRNA (Tang et al. 2007).

The modified siRNAs caused significant gene silencing as observed for the non-modified reference duplex, with the highest remaining level of expression of about 10% observed for "SIR Az-G14 as".

Conceptually, an ideal gene selected as an internal reference control should have a constant level of expression across the tissue or cell samples used throughout the experiment, and should not exhibit altered expression with diseased or, control tissues, or indeed experimental conditions [ 10].

Porcine miRNAs frequently exhibit variation from their "reference" miRBase sequences (which have the highest level of expression), producing multiple mature variants that we herein refer to as isomiRs [ 22- 24].