Reads with two different regions that mapped to separate locations along the reference were identified as chimeras.
First, each sample was aligned against an external genotype 1a reference sequence [32], and positions of variation relative to the reference were identified for each sample.
Departures from the reference were identified with GATK (11).
Departures from the reference were identified with GATK (DePristo et al., 2011).
The Korean reference was identified by the mean and standard deviation of men and women aged 20 29 years.
Reads were mapped to the UMD3.1 reference genome, and nucleotides within reads that differed from the reference were identified as SNP.
The patients' sex (with women as reference) and age (categorized by quartiles with the youngest age group as reference) were identified as independent variables at visit level.
All sequence differences between the MA ancestor, which was sequenced twice, and the reference were identified to determine the sequence of the ancestor.
Essentially, the heterozygous SNPs in the reference are identified and then used as coordinates to be examined for changes in the putative derived isolates.
No references were identified that associated the sources of the genes with allergy.
An additional 70 references were identified through manual review of the references of the studies that underwent quality assessment.
