NPHP expression performed in triplicate was normalized to GAPDH mRNA expression, and the data were analyzed with the 2−ΔΔCt method using day 2 or pSICOR samples as reference for time-course and knockdown experiments, respectively.
The results provided here for the normal heart provide an important frame of reference for time-course studies currently under way in our laboratory of the way in which perimysial collagen changes in hypertensive cardiomyopathy, postmyocardial infarction, and during development and aging.
In addition, the pools were used as references for time normalization (stretching).
The pools also served as references for time normalization (peak stretching) and were used to express the concentrations of each peak in the samples as a percentage of the concentration of those peaks in the averaged pool.
z-Scores were calculated for body weight and length/height using the 1990 British growth references for time points 6, 12, 24 and 36 months.
Among his reference points for "Time Regained" were Edward Curtis's sepia-toned, early-20th-century portraits of Native Americans, in which grandeur combines with the threat of extinction.
The CV Sepsis alert was the reference standard for time zero.
We can use this as a reference for the time that has passed.
For example Scladina was the unique available sequence around 100,000 years BP and this could influence our results because the coalescence prossess based only on one reference for this time.
In the present work, we demonstrate that FDS-SV data have inherently a reference for the time-dependent macromolecular signal intensity, resting on a geometric link between radial boundary migration and plateau signal.
