Sentence examples for reference for staining from inspiring English sources
Expression levels similar or lower than the intensity in the surrounding normal tissue (used as the internal reference for staining) were considered as low cortactin expressors and those with increased intensity as high expressors.
Sections of normal breast were used as external reference control for staining and scoring.
Inoue et al 's (1997) reference method for staining interpretation seems to be the most consistent with VEGF overexpression physiopathology: VEGF is defined as positive only whenever it is more intensely expressed in tumour cells than in normal smooth muscle cells.
Patient and healthy donor IgG were used at comparable concentration for staining, working concentrations for primary and secondary antibodies (reference anti-GAD65 monoclonal antibody: N-GAD65mAb[56], anti-calbindin, Swant) were determined empirically to ensure optimal/signal background staining.
The normal tissue serves as an internal control and a reference of staining intensity for adjacent cancer foci.
The staining obtained in the renal tissue was used as a reference for intense staining, with a score of 300.
To evaluate DHRS7 protein expression the TMAs were stained with an anti-DHRS7 antibody (rabbit anti-human DHRS7 polyclonal antibody, HPA031121; Sigma, St . Louis MO, 1 200 dilution) and analyzed by an experienced pathologist (L. T .. Reference for protein staining optimization and controls can be found at: http://www.proteinatlas.org/ENSG00000100612-DHRS7/tissue.
Using reference sections immunohistochemically stained for FasL, intratumoral variation in the level of TIL apoptosis was observed, with consistently more apoptosis of TILs in FasL-expressing tumour nests.
Using a reference section immunohistochemically stained for FasL to identify FasL-positive and FasL-negative tumour nests, we found that there were consistently fewer TILs within FasL-expressing nests relative to FasL-negative tumour nests within each tumour examined (n=16).
To evaluate the impact of FCCP treatments on the level of TMRM fluorescence in individual samples, the fluorescence intensities of reference cell populations stained for 45 min with TMRM were compared to fluorescence levels in cell populations which underwent a subsequent FCCP challenge for 20 min.
