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The OsACT1 gene (forward primer: 5'-ATGAAGATCAAGGTGGTCGC-3'; reverse primer: 5'-GTACTCAGCCTTGGCAATCC-3') was selected as an internal reference for relative quantification.
Next, the experiment was repeated with a featureless, annular gap between the disk and the reference surface (Figure 4Aii), and finally, if the subject was still able to do the task, the disk was presented on a featureless gray background with only the edges of the display screen available as a reference for relative depth (Figure 4Aiii).
The laboratorial strain S288C was used as reference for relative genome profiling.
Cr is generally considered to be a stable metabolite; many studies apply Cr as reference for relative metabolite quantification.
The reaction product served both as a control for RT PCR performance and as a reference for relative quantification.
VDAG_05595 was therefore used as a reference for relative gene expression analyses in accordance with Pfaffl et al. [ 58].
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References for relative transcript levels were set to 1.
RPL37A (Hs01102345_m1), which is the optimal reference for meningiomas and control tissues (Pfister et al, 2011), was used as the reference gene for relative quantification.
To identify an appropriate reference gene for V. dahliae MS + and NoMS cultures, the RNA-seq data were examined for potential reference genes for relative gene expression.
We have redressed the situation by identifying reference genes for relative quantification of transcripts in rice under drought studies, taking into consideration tissues, genotypes and growth stages.
In conclusion, we identified reference genes for relative quantification of transcript across rice samples under drought conditions, according to their broad expression stability under different variables.
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