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Exact Green׳s functions for the Helmholtz equation are first reviewed and implemented in a quiescent propagation space from reference expressions of the literature.
For each region (cytoband), a general test (such as binomial or t-test) is applied to determine if the gene expressions in the region are collectively higher or lower than the reference expressions.
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Unless a stably expressed gene is used as a reference, expression level in samples are overestimated or underestimated, and identification of differential expressions is prone to errors.
Based on ratios of transcript quantities to the constitutively expressed EF1α gene used as a reference, expression levels of the 13 METC genes were significantly (P < 0.01) lower in RfBP+ than in WT plants.
This formalization gives a reference expression of AADL semantics which can be compared with the TASM-based translation (for verification purpose).
Genevestigator is a reference expression database and was chosen as an analysis tool because it contains high quality manually assessed data (Grennan, 2006).
Therefore, BestKeeper is highly recommendable to select the most stable gene to be used as internal standard and the selection of a specific reference expression gene requires a validation for each tissue and experimental design.
Then reference expression stability measure (M value) is calculated with the average of pairwise variations.
The former consists of two haplotypes both with the reference expression phenotype.
As shown, then, the haplotypes segregate into three groups: reference expression and downregulation, increased expression with reference downregulation, and increased expression with increased downregulation.
* Log10Ratio: logarithm (base 10) of the ratio between PC sample and prostate reference expression values.
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