Sentence examples for reference expression levels of from inspiring English sources

Based on ratios of transcript quantities to the constitutively expressed EF1α gene used as a reference, expression levels of the 13 METC genes were significantly (P < 0.01) lower in RfBP+ than in WT plants.

When compared to the genomic DNA reference, expression levels of most genes in group 1 were high relative to the normalized mid-level of the reference, while most of those in group 2 were low (Fig. 1A, Additional file 3).

The ability to cross-reference expression levels of EGFRvIII and other RTK deletions against the clinical and detailed molecular data in TCGA provides a valuable resource to better understand the molecular context in which these mutations are found.

Assays were done following manufacture's instructions and using as reference the expression levels of 18S.

For instance when the low expression group of genes is used as a reference, the expression levels of high expression genes within handled or high activity mice were increased on average by greater than 1.5-fold.

Although we did not synchronously quantify the expression level of miRNAs with their targets, we referenced the expression levels of these miRNAs in the two libraries (ZJ and ZJ-COLD) and found that bxy-novel-10, bxy-mir-29 and bxy-mir-40 were down-regulated in the ZJ-COLD library, with bxy-mir-29 down regulation the more obvious.

Relative quantitation and normalization of the amplified targets were performed by the comparative ΔΔCt method in reference to the expression levels of the housekeeping gene ubiquitin [ 69].

To select the best reference gene, the expression levels of the twelve genes were measured in 32 glioma samples using real-time PCR.

By mapping the resulting short sequencing reads onto the reference genome, the expression levels of genes relative to the condition of interest or absolute levels can be quantified [ 9, 11].

Using mRNA levels inherent to MSCs as a reference, the relative expression levels of the corresponding genes in the hepatocyte-like cells, HepG2 cells and the primary human hepatocytes were determined using quantitative real-time PCR.

Samples were normalized to the GAPDH reference gene and relative expression levels of all genes were determined using the ΔΔCt method.