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Unless a stably expressed gene is used as a reference, expression level in samples are overestimated or underestimated, and identification of differential expressions is prone to errors.
Based on ratios of transcript quantities to the constitutively expressed EF1α gene used as a reference, expression levels of the 13 METC genes were significantly (P < 0.01) lower in RfBP+ than in WT plants.
Genevestigator is a reference expression database and was chosen as an analysis tool because it contains high quality manually assessed data (Grennan, 2006).
This formalization gives a reference expression of AADL semantics which can be compared with the TASM-based translation (for verification purpose).
Therefore, BestKeeper is highly recommendable to select the most stable gene to be used as internal standard and the selection of a specific reference expression gene requires a validation for each tissue and experimental design.
During optimization, a separate cell line, RAMOS (RA-1 CRL1596, ATCC) was used to independently confirm the specificity of the primary antibodies (CD34, CD45, CD71 and CD235a) compared to K562 cells (using ATCC reference expression levels and those determined in our lab) and HSC-derived erythroid cells.
Similar(22)
Exact Green׳s functions for the Helmholtz equation are first reviewed and implemented in a quiescent propagation space from reference expressions of the literature.
For each region (cytoband), a general test (such as binomial or t-test) is applied to determine if the gene expressions in the region are collectively higher or lower than the reference expressions.
The ability to cross-reference expression levels of EGFRvIII and other RTK deletions against the clinical and detailed molecular data in TCGA provides a valuable resource to better understand the molecular context in which these mutations are found.
Genes in the HI2424 genome were linked to the AU1054 genes used as a reference in this study using MUMmer 3.0 (Kurtz et al. 2004), which provided data that were read into R (R Development Core Team 2011) to link and cross-reference expression values between genomes.
Gene is a description of the gene function, contig references expression in table 2, sense is the forward primer, antisense is the reverse primer and efficiency is the efficiency of the primer pair in qPCR.
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