Exact(1)
A modified DST reading schedule for such samples, with a final reading 1 week after a MODS culture turns positive, marginally improves the concordance with reference DST.
Similar(59)
The results of the reference proportion DST method, defined as susceptible or resistant, were available to clinicians for patient care.
For our primary analysis using the culture-based DST reference standard, we performed sensitivity analyses for four QUADAS-2 signalling questions to explore whether the results we found were robust with respect to the methodological quality of the studies.
We planned to investigate heterogeneity in relation to the reference standard (culture-based DST compared with (1) genetic sequencing, (2) culture-based DST and genetic sequencing, and (3) culture-based DST followed by genetic sequencing with discordant results) and individual drugs within a drug class (for example, ofloxacin and moxifloxacin within the FQ class).
A round of proficiency testing by the supranational TB reference laboratories showed that DST for rifampicin presented difficulties in correctly identifying resistance in these top level laboratories [ 44].
We also determined MTBDR sl accuracy using genetic testing (gene sequencing of loci known to be associated with drug resistance) and both genetic testing and culture-based DST as reference standards.
The gold-standard reference test was the DST-LJ result.
DST refers to the developmental stage tissue.
Reference standard is culture-based DST followed by genetic sequencing of discrepant index test-culture-based DST results: We found a single study (Miotto 2012) that used phenotypic culture-based DST and performed genetic testing only on discrepant results.
Reference standard is culture-based DST followed by genetic sequencing of discrepant index test-culture-based DST results: For individual studies (three in total), sensitivity estimates ranged from 73%to100%0% and specificity estimates ranged from 94%to100%0%.
Reference standard is culture-based DST followed by genetic sequencing of discrepant index test-culture-based DST results: No studies performed indirect MTBDR sl testing when performed indirectly for XDR-TB and used phenotypic culture-based DST and genetic sequencing for discordant analysis as a reference standard.
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