Sentence examples for reference construct from inspiring English sources
(ii) Secondly, apart from quantitative deviations in the kbi,ratio the Head3/Neck1 chimera showed fundamental differences to the NcKin3 reference construct.
ATP-dependent ADP release from the single-head attached intermediate does not occur in the wildtype NcKin3 reference construct whose second passive head does not show ATP-dependent interaction with microtubules [16].
In microscopic motility assays, this construct never showed any typical indications of processive motility: characteristic pivoting at low motor densities was not observed, microtubules moved farther than their length, and in contrast to the NcKin3 reference construct the chimera was not accelerated by higher motor coating densities.
The cells containing FSI, replaced at signal and leader peptide region, led to high F3′5′H activity in the range of 39.26 pkat · L−1 culture, a significant increase in comparison with the reference construct (yCsF3′5′H).
We prepared a reference construct by digesting the HSV-TK promoter, between the BamH1 site and Hind III sites, from the pRL-TK vector (Promega Corporation, Madison, WI, USA) that expresses Renilla luciferase, and cloning this fragment into the pGL4.18 [ luc2p/Neo] vector (Promega) that expresses Firefly luciferase.
Novoalign uses a degenerate reference constructed by replacing the allele in the reference sequence with the corresponding genetic ambiguity code (IUPAC) at HSV sites.
Maq-120 alignment was also performed on the component of reads that failed to map to the NCBI Unigene reference against a new reference constructed from a 5× assembly of wheat Chinese Spring genomic 454 reads (M. Bevan, JIC, personal communication).
Optimal growth monitoring requires up-to-date reference growth data on representative samples from the population and growth references constructed according to the state-of-the-art statistical methodologies [ 5].
Wildtype reference constructs were prepared as described [40].
On average, background values were <1% of the values obtained for reference constructs.
To identify the functional consequences the domain swaps, we tested the mutants along with wildtype reference constructs in motility assays.
